【英文标准名称】:Microbiologyoffoodandanimalfeedingstuffs-Horizontalmethodfortheenumerationofbeta-glucuronidase-positiveEscherichiacoli-Part3:Mostprobablenumbertechniqueusing5-bromo-4-chloro-3-indolyl--D-glucuronide
【原文标准名称】:食品和动物饲料的微生物学.β葡糖苷酸酶阳性大肠杆菌记数的水平方法.第3部分:用5-溴-4-氯3-吲哚-β-D葡糖苷酸的莫斯随机计数技术
【标准号】:ISO/TS16649-3-2005
【标准状态】:现行
【国别】:国际
【发布日期】:2005-09
【实施或试行日期】:
【发布单位】:国际标准化组织(IX-ISO)
【起草单位】:ISO/TC34
【标准类型】:()
【标准水平】:()
【中文主题词】:试验报告;试样;肉;微生物学;验证;定义;试验;微生物;食品;食品检验;水平的;抽样方法;肉制品;大肠杆菌;;试样制备;计数;饲料
【英文主题词】:Animalfeed;Biochemicalmethodsofanalysis;Colibacteria;Definitions;Enumerations;Escherichiacoli;Foodinspection;Foodproducts;Horizontal;Investigations;Meat;Meatproducts;Microbiologicalanalysis;Microbiology;Micro-organisms;Microorganisms;Samples;Samplingmethods;Specimenpreparation;Testing;Verification
【摘要】:ThisTechnicalSpecificationspecifiesahorizontalmethodfortheenumerationofβ-glucuronidase-positiveEscherichiacoli,bymeansoftheliquid-mediumculturetechniqueandcalculationofthemostprobablenumber(MPN)afterincubationat37℃,thenat44℃.ThisTechnicalSpecificationisapplicableto—productsintendedforhumanconsumptionandthefeedingofanimals,and—environmentalsamplesintheareaoffoodproductionandfoodhandling.ThemethodissuitablefortheenumerationofcellsofEscherichiacolithatmayhavebeensubjectedtostressarisingfromdehydration,freezing,exposuretoasaline(suchasmarine)environmentordamagebydisinfectantssuchaschlorine-containingproducts.AlimitationoftheapplicabilityofthisTechnicalSpecificationisimposedbythesusceptibilityofthemethodtoalargedegreeofvariability.ThemethodistobeappliedandtheresultsinterpretedinthelightoftheinformationgiveninClause11.WARNING—StrainsofEscherichiacolithatdonotgrowat44℃and,inparticular,thosethatareβ-glucuronidasenegative,suchasEscherichiacoliO157andsomeotherstrainsofpathogenicE.coli,willnotbedetectedbythemethoddescribedinthisTechnicalSpecification.
【中国标准分类号】:C53
【国际标准分类号】:07_100_30
【页数】:8P.;A4
【正文语种】:英语